Journal: Current Research in Food Science
Article Title: Lacticaseibacillus rhamnosus LRJ-1 alleviates constipation through promoting gut Bacteroides -derived γ-aminobutyric acid production
doi: 10.1016/j.crfs.2024.100924
Figure Lengend Snippet: L. rhamnosus LRJ-1 elevated Bacteroides level in the gut microbiota and the level of various enzymes. (A and B) Boxplots of alpha diversity indices of bacterial community, include Shannon (A) and Simpson (B) indices. (C) PLS-DA score plot of bacterial community in Con, Lop and LRJ-1 groups. (D) Change in genus with different treatments. (E) The log-transformed LDA scores computed with LEfSe for bacterial taxa differentially abundant between LRJ-1 and Lop groups. Positive and negative LDA score indicates enrichment in LRJ-1 and Lop group, respectively. (F) Correlation between GABA and microbes was estimated by Spearman's correlation analysis. (G) Representation of GABA synthesis related metabolic pathways identified from metagenomics data. Pathways include GABA shunt, arginine biosynthesis, threonine biosynthesis and TCA cycle. (H) The abundance difference of enzyme genes involved in GABA shunt, arginine biosynthesis, threonine biosynthesis and TCA cycle pathway between LRJ-1 and Lop groups was analyzed by STAMP. Bioinformatic analysis was performed with the OmicStudio tool at https://www.omicstudio.cn/tool and software STAMP v2.1.3.
Article Snippet: As B . uniformis was the most enriched Bacteroides species ( E) caused by L. rhamnosus LRJ-1 treatment, a B . uniformis ATCC 8492 (BU) strain was used to investigate its role in alleviating constipation.
Techniques: Transformation Assay, Software